Coding

Part:BBa_K4415005:Design

Designed by: William Pihlainen-Bleecker   Group: iGEM22_McMaster_A   (2022-09-29)


Human codon optimized eGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 576
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amino acid sequence was codon optimized for expression in human cells using IDT's tool.


Source

Amino acid sequence taken from pAAV-GFP: https://www.addgene.org/32395/

References